DNA Bank
Welcome to the UCSF DNA Bank. The DNA Bank is a unit within the UCSF Genomics Core Facility. The DNA Bank is located at the Mission Bay Campus, Rock Hall, Room 515. A picture ID is required for entrance to the building.
The DNA Bank accepts the clinical samples from projects that have been approved by the Committee on Human Research (CHR).
Genomic DNA isolation is performed utilizing standardized and quality controlled Gentra Systems' PureGene DNA isolation system or Qiagen Kits.
The quantity and quality of the genomic DNA isolate is determined by 260/280 UV spectrophotometery. At regular intervals, the integrity of isolated DNA is evaluated by agarose gel electrophoresis performed on randomly selected isolates.
Following isolation and quality assessment, DNA can be aliquoted into a normalized concentration. The preparation of aliquots serves to allow ready distribution of DNA samples to both the client laboratory and their collaborators and to preclude excessive routine freezing and thawing of the primary DNA isolate, a practice which is well known to result in notable degradation of genomic DNA stocks.
All samples are stored in alarmed Revco ultra-low freezers at -80ºC. All of the ultra-low freezer units utilized by the DNA Bank are monitored by a temperature sensitive alarm system that provides 24 hour oversight. In the event of a power outage, all freezers are on an emergency back-up electrical generator.
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